WheatExp: an RNA-seq expression database for polyploid wheat.

BACKGROUNDFor purposeful genomics research, you will need to perceive the dynamic expression profiles of transcribed genes in numerous tissues, phases of improvement and in response to environmental stimuli.

The proliferation in using next-generation sequencing applied sciences by the plant analysis group has led to the buildup of huge volumes of expression information. However, evaluation of those datasets is difficult by the frequent incidence of polyploidy amongst economically-important crop species. In addition, processing and analyzing such massive volumes of sequence information is a technical and time-consuming job, limiting their software in purposeful genomics research, significantly for smaller laboratories which lack entry to high-powered computing infrastructure.

Wheat is an efficient instance of a younger polyploid species with three related genomes (97 % similar amongst homoeologous genes), quickly accumulating RNA-seq datasets and a big analysis group.METHODSWe current WheatExp, an expression database and visualization instrument to investigate and evaluate homoeologue-specific transcript profiles throughout a broad vary of tissues from totally different developmental phases in polyploid wheat. Beginning with publicly-available RNA-seq datasets, we developed a pipeline to differentiate between homoeologous transcripts from annotated genes in tetraploid and hexaploid wheat.

Data from a number of research is processed and compiled right into a database which may be queried both by BLAST or by looking for a recognized gene of curiosity by title or purposeful area. Expression information of a number of genes may be displayed side-by-side throughout all expression datasets offering instant entry to a complete panel of expression information for particular subsets of wheat genes.

The introduced expression information is homoeologue-specific permitting for the evaluation of relative contributions from every genome to the general expression of a gene, a important consideration for breeding functions. Our strategy may be expanded to different polyploid species by adjusting sequence mapping parameters based on the particular divergence of their genomes.

WheatExp: an RNA-seq expression database for polyploid wheat.
WheatExp: an RNA-seq expression database for polyploid wheat.

CytoWatch™ Multiplexing Buffer for Flow Cytometry *100X*

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Rat mesenchymal Stem cell Flow Cytometry Kit

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Human mesenchymal Stem cell Flow Cytometry Kit

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Mouse mesenchymal Stem cell Flow Cytometry Kit

MUXMX-09011 10 tests
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CytoWatchâ„¢ Multiplexing Buffer for Flow Cytometry *20X*

37010-100Tests 100 Tests
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Description: Fluorescent antibody conjugates tend to aggregate when in close contact, distorting the analytical results of multicolor staining cells due to FRET and other energy transfer phenomena between different conjugates.

CytoWatchâ„¢ Multiplexing Buffer for Flow Cytometry *20X*

37011-1000Tests 1000 Tests
EUR 308
Description: Fluorescent antibody conjugates tend to aggregate when in close contact, distorting the analytical results of multicolor staining cells due to FRET and other energy transfer phenomena between different conjugates.

Goat IgG (-ve control for flow cytometry) (isotype control)

20011-100 100 test
EUR 123.6

ExoFlow-ONE EV Labeling Kit for Flow Cytometry (Ruby Red)

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Annexin Binding Buffer *10X Optimized for Imaging and Flow Cytometry*

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Human RAC1 AssayLite RPE-Conjugated Antibody Flow Cytometry KitKit

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Cell Meterâ„¢ Intracellular GSH Assay Kit *Optimized for Flow Cytometry*

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Description: Our Cell Meter™ assay kits are a set of tools for monitoring cell viability.

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Cell Meter™ Intracellular GSH Assay Kit *Optimized for Flow Cytometry*

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Description:

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Cell Meterâ„¢ FITC-Annexin V Binding Apoptosis Assay Kit *Optimized for Flow Cytometry*

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Cell Meter™ FITC-Annexin V Binding Apoptosis Assay Kit *Optimized for Flow Cytometry*

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Description: Our Cell Meter™ assay kits are a set of tools for monitoring cell viability.

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Cell Meter™ Intracellular GSH Assay Kit *Optimized for Flow Cytometry with 405 nm excitation*

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Cell Meter™ Fluorimetric Intracellular Peroxynitrite Assay Kit *Optimized for Flow Cytometry*

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Human Thioredoxin-1 (TRX1) AssayLite Multi-Color Conjugated Antibodies Flow Cytometry Kit

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Description: Our Cell Meter™ assay kits are a set of tools for monitoring cell viability.

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Description: Our Cell Meter™ assay kits are a set of tools for monitoring cell viability.

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Cell Meterâ„¢ JC-10 Mitochondrion Membrane Potential Assay Kit *Optimized for Flow Cytometry Assays*

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Cell Meterâ„¢ Annexin V Binding Apoptosis Assay Kit *Green Fluorescence Optimized for Flow Cytometry*

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Description: Our Cell Meter™ assay kits are a set of tools for monitoring cell viability.

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Description: Our Cell Meter™ assay kits are a set of tools for monitoring cell viability.

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Description: Our Cell Meter™ assay kits are a set of tools for monitoring cell viability.

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Description: Our Cell Meter™ assay kits are a set of tools for monitoring cell viability.

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Description: Our Cell Meter™ assay kits are a set of tools for monitoring cell viability.

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Cell Meterâ„¢ Generic Fluorimetric Caspase Binding Assay Kit *Red Fluorescence Optimized for Flow Cytometry*

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Description: Our Cell Meter™ assay kits are a set of tools for monitoring cell viability.

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Cell Meter™ Annexin V Binding Apoptosis Assay Kit *Deep Red Fluorescence Optimized for Flow Cytometry*

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Cell Meterâ„¢ Mitochondrion Membrane Potential Assay Kit *Orange Fluorescence Optimized for Flow Cytometry*

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Description: This particular kit is designed to detect cell apoptosis by measuring the loss of the mitochondrial membrane potential(MMP).

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Human ATP Synthase Subunit O, Mitochondrial (ATP5O) AssayLite FITC-Conjugated Antibody Flow Cytometry Kit

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Cell Meterâ„¢ Generic Fluorimetric Caspase Activity Assay Kit *Green Fluorescence Optimized for Flow Cytometry*

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Description: Our Cell Meter™ assay kits are a set of tools for monitoring cell viability.

Pyruvate Dehydrogenase Complex (PDH Complex) AssayLite Multi-Color Conjugated Antibodies Flow Cytometry Kit

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Cell Meterâ„¢ Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Blue Fluorescence Optimized for Flow Cytometry*

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Description: Hydrogen peroxide is a reactive oxygen metabolic by-product that serves as a key regulator for a number of oxidative stress-related events.

Human Acyl-CoA Synthetase Family Member 2 (ACSF2) AssayLite Multi-Color Conjugated Antibodies Flow Cytometry Kit

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Cell Meter™ Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Green Fluorescence Optimized for Flow Cytometry*

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Cell Meter™ Fluorimetric Intracellular Nitric Oxide (NO) Activity Assay Kit *Red Fluorescence Optimized for Flow Cytometry*

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Cell Meterâ„¢ Fluorimetric Intracellular Nitric Oxide (NO) Activity Assay Kit *Orange Fluorescence Optimized for Flow Cytometry*

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Description: Nitric oxide (NO) is an important biological regulator involved in numbers of physiological and pathological processes.

Cell Meterâ„¢ Fluorimetric Intracellular Nitric Oxide (NO) Activity Assay Kit *Red Fluorescence Optimized for Flow Cytometry*

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Cell Meterâ„¢ Flow Cytometric Calcium Assay Kit

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