RNA Binding Protein (RBP) Expression and Disease Dynamics database (READ DB) is a non-redundant, curated database of human RBPs. RBPs curated from completely different experimental research are reported with their annotation, tissue-wide RNA and protein expression ranges, evolutionary conservation, disease associations, protein-protein interactions, microRNA predictions, their identified RNA recognition sequence motifs in addition to predicted binding targets and related purposeful themes, offering a one cease portal for understanding the expression, evolutionary trajectories and disease dynamics of RBPs within the context of post-transcriptional regulatory networks.
Lung adenocarcinoma (LUAD), a type of lung most cancers, is reported to trigger first and second-order most cancers morbidity to males and ladies in China, respectively. We assessed the mRNA expression of GJB2 in LUAD sufferers in our examine, based mostly on information acquired from the most cancers genome atlas (TCGA) and in order to extend additional information into the organic pathways concerned in LUAD pathogenesis associated to GJB2.Information on gene expression and evaluating medical information had been acknowledged and downloaded from TCGA.
Gene set enrichment evaluation (GSEA) created an organized checklist of all genes is indicated by their reference to GJB2 expression.Our examine cohort included 265 (54.5%) feminine and 221 (36.0%) male sufferers. The scatter plot and paired plot confirmed the distinction of GJB2 expression between regular and tumor samples (P < .01).
Overall survival (OS) evaluation demonstrated that LUAD with GJB2 -high had a extra horrible prognosis than that with GJB2 -low (P < .01). Multivariate evaluation with the cox proportional hazards mannequin indicated that the expression of Cx26 (HR: 1.00; 95%CI: 1.00-1.01; P = .041) and stage (HR: 1.95; 95%CI: 1.23-3.09; P = .003) had been unbiased prognostic elements for sufferers with LUAD.
The GSEA outcomes confirmed that cytosolic DNA sensing pathway, apoptosis, cytokine-cytokine receptor interplay, pure killer cell mediated cytotoxicity, regulation of actin cytoskeleton, toll-like receptor signaling pathway, small cell lung most cancers and pathways in most cancers are differentially enriched in GJB2 excessive expression phenotype.
Database of RNA binding protein expression and disease dynamics (READ DB).
Our examine confirmed the considerably excessive ranges of Cx26 expression in LUAD sufferers with a number of noticed medical options. GJB2 could also be a probably helpful prognostic molecular biomarker of unhealthy survival in LUAD, whereas additional experimental must be carried out to exhibit the biologic impact of GJB2.
Multiplex Human Cytokine ELISA Kit (M1/M2/MDSC Cytokines)
The Multiplex ELISA Kit For Human Cytokine Panel 2 (4-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing concurrent measurement of four biomarkers or analytes (IL-1α, IL-4, IL-6, TNFα).
Each kit contains a 96-well plate, with each well featuring the relevant biomarker panel, and all the reagents required to perform testing. Multiplex plates are built by absorbing four distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results.
Using just 25 µl of sample per well, up to 80 samples can be assayed for all four markers in the panel in 2.25 hours. Multiplex ELISA Kit For Human Cytokine Panel 2 (4-Plex) provides scientists with an easy-to-use and cost-effective means of generating a cytokine profile for each sample.
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Assay Ranges
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Analyte
Assay Type
Calibrator Range
Upper Limit of Quantification (ULOQ)
Lower Limit of Quantification (LLOQ)
Limit of Detection
IL-1α
Sandwich
4000 -5.49 (pg/mL)
2800 (pg/mL)
7.1 (pg/mL)
5.43 (pg/mL)
IL-4
Sandwich
1676 – 2.30 (pg/mL)
1173 (pg/mL)
3.0 (pg/mL)
1.79 (pg/mL)
IL-6
Sandwich
3000 – 4.12 (pg/mL)
2100 (pg/mL)
5.3 (pg/mL)
3.47 (pg/mL)
TNFα
Sandwich
6500 – 8.92 (pg/mL)
4522 (pg/mL)
11.5 (pg/mL)
7.74 (pg/mL)
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Kit Components
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Each kit contains a 96-well plate, featuring the relevant biomarker panel in each well, and all reagents required to perform testing.
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Reagents Include:
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Calibrator: Lyophilized, recombinant antigens in a buffered protein base;_x000D_Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives;_x000D_Sample Diluent: Liquid, 10 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives;_x000D_Streptavidin HRP (1X): Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase;_x000D_Substrate A: Liquid, 3mL/vial of stabilized hydrogen peroxide;Substrate B: Liquid, 3 mL/vial of stabilized signal enhancer;_x000D_Wash Buffer Concentrate (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant;Plate Seals (3): Adhesive strips
Multiplex ELISA Kit For Mouse Cytokine Panel 3 (4-Plex)
The Multiplex ELISA Kit For Mouse Cytokine Panel 3 (4-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing concurrent measurement of four biomarkers or analytes (IL-2, IL-4, IL-6, TNFα).
Each kit contains a 96-well plate, with each well featuring the relevant biomarker panel, and all the reagents required to perform testing. Multiplex plates are built by absorbing four distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results.
Using just 25 µl of sample per well, up to 80 samples can be assayed for all four markers in the panel in 5.5 hours. Multiplex ELISA Kit For Mouse Cytokine Panel 3 (4-Plex) provides scientists with an easy-to-use and cost-effective means of generating a cytokine profile for each sample.
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Assay Ranges
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Analyte
Assay Type
Calibrator Range
Upper Limit of Quantification (ULOQ)
Lower Limit of Quantification (LLOQ)
Limit of Detection
IL-2
Sandwich
360 - 0.49 (pg/mL)
252 (pg/mL)
0.6 (pg/mL)
0.25 (pg/mL)
IL-4
Sandwich
400 - 0.55 (pg/mL)
280 (pg/mL)
0.7 (pg/mL)
0.27 (pg/mL)
IL-6
Sandwich
504 - 0.69 (pg/mL)
353 (pg/mL)
0.91 (pg/mL)
0.35 (pg/mL)
TNFα
Sandwich
429 - 0.59 (pg/mL)
300 (pg/mL)
0.69 (pg/mL)
0.29 (pg/mL)
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Kit Components
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Each kit contains a 96-well plate, featuring the relevant biomarker panel in each well, and all reagents required to perform testing.
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Reagents Include:
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Calibrator: Lyophilized, recombinant antigens in a buffered protein base;_x000D_Detection Mix: Lyophilized, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives;_x000D_Sample Diluent: Liquid, 10 mL/vial of a buffered protein solution;_x000D_Substrate A: Liquid, 3.5 mL/vial;_x000D_Substrate B+: Liquid, 3.5 mL/vial;_x000D_Streptavidin-HRP: Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase;_x000D_Wash Buffer Concentrate (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant;_x000D_Plate Seals (3): Adhesive strips
Multiplex ELISA Kit For Rat Cytokine - Inflammation (9-plex)
Description: T helper cells (Th) play a vital role in the adaptive immune system by suppressing or regulating immune responses. After Th actived, it will differentiate into T-helper 1 (Th1) or T-helper 2 (Th2) effector cells. These two subsets are characterized by their distinct cytokine expression pattern and the immune function they mediate. Th1 and Th2 cells each produce a specialized combination of cytokines that depends on type of pathogen that the helper T cell has recognized—some cytokines are more effective than others in the process of eliminating certain invaders. In general, TH1 cytokines play the role of immune killing, while Th2 cytokines play the role of immune protection. Th1/Th2 paradigm provides rationale for developing new vaccines against infectious diseases and novel strategies for the therapy of allergic and autoimmune disorders.
Human Th1/Th2 Cytokine Kit (Flow Cytometry Multiplex Bead Assay)
Description: T helper cells (Th) play a vital role in the adaptive immune system by suppressing or regulating immune responses. After Th actived, it will differentiate into T-helper 1 (Th1) or T-helper 2 (Th2) effector cells. These two subsets are characterized by their distinct cytokine expression pattern and the immune function they mediate. Th1 and Th2 cells each produce a specialized combination of cytokines that depends on type of pathogen that the helper T cell has recognized—some cytokines are more effective than others in the process of eliminating certain invaders. In general, TH1 cytokines play the role of immune killing, while Th2 cytokines play the role of immune protection. Th1/Th2 paradigm provides rationale for developing new vaccines against infectious diseases and novel strategies for the therapy of allergic and autoimmune disorders.
Multiplex ELISA Kit For Human Cytokine High Sensitivity (4-Plex)
The Multiplex ELISA Kit For Human Cytokine High Sensitivity (4-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing concurrent measurement of four biomarkers or analytes (IL-4, IL-6, IL-10, IFNγ).
Each kit contains a 96-well plate, with each well featuring the relevant biomarker panel, and all the reagents required to perform testing. Multiplex plates are built by absorbing four distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results.
Using just 25 µl of sample per well, up to 80 samples can be assayed for all four markers in the panel in 2.25 hours. Multiplex ELISA Kit For Human Cytokine High Sensitivity (4-Plex) provides scientists with an easy-to-use and cost-effective means of generating a cytokine profile for each sample.
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Assay Ranges
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Analyte
Assay Type
Calibrator Range
Upper Limit of Quantification (ULOQ)
Lower Limit of Quantification (LLOQ)
Limit of Detection
IFNγ
Sandwich
99 – 0.14 (pg/mL)
69.3 (pg/mL)
0.18 (pg/mL)
0.10 (pg/mL)
IL-4
Sandwich
21 – 0.03 (pg/mL)
14.7 (pg/mL)
0.04 (pg/mL)
0.02 (pg/mL)
IL-6
Sandwich
333 – 0.46 (pg/mL)
233 (pg/mL)
0.59 (pg/mL)
0.20 (pg/mL)
IL-10
Sandwich
2680 – 3.68 (pg/mL)
1876 (pg/mL)
4.78 (pg/mL)
2.98 (pg/mL)
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Kit Components
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Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip-well format, please contact us for customization.
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Reagents include:
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Wash Buffer Concentrate (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant;_x000D_Sample Diluent: Liquid, 10 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives;_x000D_Detection Mix: Lyophilized, biotinylated antibodies in a buffered protein solution with preservatives. Reconstitute to 6 mL with Ultra Pure Lab Water;_x000D_Calibrator: Lyophilized, recombinant antigens in a buffered protein base;_x000D_Streptavidin-HRP (1X): Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase;_x000D_Substrate A: Liquid, 3 mL/vial of stabilized hydrogen peroxide;_x000D_Substrate B+: Liquid, 3 mL/vial of stabilized signal enhancer;_x000D_Plate Seals (3): Adhesive strips
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Multiplex ELISA Kit For Human Cytokine Release Syndrome (16-Plex)
The Multiplex ELISA Kit For Human Cytokine Release Syndrome (16-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of 16 biomarkers or analytes. Using only 25 µl of sample per well, up to 80 samples can be assayed for all 16 markers in the panel within 4.83 hours.
The Multiplex ELISA Kit For Human Cytokine Release Syndrome (16-Plex) provides researchers an easy to use and cost-effective means of generating a cytokine profile for each sample. Multiplex plates are built by adsorbing 16 distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results.
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Assay Ranges
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Analytes
Calibrator Range
Units
LLD
GMCSF
6,000-10.70
pg/mL
5.35
IFNα
400-0.72
pg/mL
0.36
IFNγ
99-0.18
pg/mL
0.09
IL-1β
4,075-7.27
pg/mL
3.64
IL-1RA
7,000-12.48
pg/mL
6.24
IL-2
64-0.11
pg/mL
0.06
IL-2Rα
16,000-28.5
pg/mL
14.25
IL-6
333-0.59
pg/mL
0.3
IL-6R
1,250-2.23
pg/mL
1.11
IL-8
2,000-3.6
pg/mL
1.8
IL-10
2,680-4.78
pg/mL
2.39
IL-12p70
3,000-5.35
pg/mL
2.68
IL-13
333-0.59
pg/mL
0.3
MCP-1
2,000-3.6
pg/mL
1.8
MIP-1α
2,000-3.57
pg/mL
1.78
TNFα
661-1.18
pg/mL
0.59
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Kit Components
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Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing.
_x000D_
Reagents include:
_x000D_
Wash Buffer Concentrate (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant;_x000D_Sample Diluent: Liquid, 10 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives;_x000D_Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered solution with preservatives;_x000D_Calibrator: Lyophilized, recombinant antigens in a buffered protein base;_x000D_Streptavidin-HRP (1X): Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase;_x000D_Substrate A: Liquid, 3 mL/vial of stabilized hydrogen peroxide;_x000D_Substrate B+: Liquid, 3 mL/vial of stabilized signal enhancer;_x000D_Plate Seals (3): Adhesive strips
Description: T helper cells (Th) play a vital role in the adaptive immune system by suppressing or regulating immune responses. After Th actived, it will differentiate into T-helper 1 (Th1) or T-helper 2 (Th2) effector cells. These two subsets are characterized by their distinct cytokine expression pattern and the immune function they mediate. Th1 and Th2 cells each produce a specialized combination of cytokines that depends on type of pathogen that the helper T cell has recognized—some cytokines are more effective than others in the process of eliminating certain invaders. In general, TH1 cytokines play the role of immune killing, while Th2 cytokines play the role of immune protection. Th1/Th2 paradigm provides rationale for developing new vaccines against infectious diseases and novel strategies for the therapy of allergic and autoimmune disorders.
The Multiplex ELISA Kit For Human Malaria (5-Plex) is a robust and fully quantitative chemiluminescent assay for the concurrent surveillance of four malaria biomarkers. The assay simultaneously measures HRP2, LDH-Pan, LDH-Pv, LDH-Pf, and one host biomarker, CRP. The monitoring of subclinical Malaria infections and prevalence of HRP2-deletions is achieved by the coupling of the most sensitive commercially available HPR2 assay with LDH detection. Validated protocols for whole blood samples are in place, with protocols for dry blood spots in development.
Each kit contains a 96-well plate and all other reagents necessary to perform the assay. The individual wells of the plate are precisely and reproducibly arrayed with each of the five biomarkers for accurate results.
In 3.5 hours, each kit will report quantities of 80 samples for the four Malaria biomarkers and one host biomarker CRP. This is achieved while only using 15 μl of whole blood per sample.
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Assay Ranges
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Analyte
Assay Type
Calibrator Range
Upper Limit of Quantification (ULOQ)
Lower Limit of Quantification (LLOQ)
Limit of Detection
CRP
Competitive
62,800 – 61 (ng/mL)
11,125 (ng/mL)
60 (ng/mL)
30.5 (ng/mL)
HRP2
Sandwich
950 – 0.9 (pg/mL)
675 (pg/mL)
0.92 (pg/mL)
0.45 (pg/mL)
LDH -Pan
Sandwich
70,750 – 69 (pg/mL)
17,687.5 (pg/mL)
24 (pg/mL)
34.5 (pg/mL)
LDH-Pf
Sandwich
22,500 - 22 (pg/mL)
5,750 (pg/mL)
8 (pg/mL)
11 (pg/mL)
LDH-Pv
Sandwich
43,750 – 43 (pg/mL)
5,000 (pg/mL)
6 (pg/mL)
21.5 (pg/mL)
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Kit Components
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Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip-well format, please contact us for customization.
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Reagents include:
_x000D_
Wash Buffer Concentrate (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant;_x000D_Assay Diluent: Liquid, 6 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives;_x000D_Calibrator Diluent: Lyophilized, buffered protein solution containing preservatives;_x000D_Competitor: Lyophilized, biotinylated competitive antigen;_x000D_Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives;_x000D_Calibrator: Lyophilized, recombinant antigens in a buffered protein base;_x000D_Streptavidin-HRP (1X): Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase;_x000D_Substrate A+: Liquid, 3 mL/vial of stabilized hydrogen peroxide;_x000D_Substrate B: Liquid, 3 mL/vial of stabilized signal enhancer;_x000D_Plate Seals (3): Adhesive strips
Description: Cytokines play an important role in physiological and pathological processes such as immune regulation, inflammatory response and tumor metastasis. Cytokine detection was wildly used in infectious diseases, autoimmune diseases, tumor auxiliary diagnosis, disease evaluation, medication guidance and prognosis judgment. Multiple cytokines can be detected by the flow cytometry multiplex bead assay at the same time, and multiple results can be obtained in a single sample test at once time, reducing samples and reagents using. The compatible flow cytometers are universal and simple to operate.
Human inflammation Kit (3-plex) (Flow Cytometry Multiplex Bead Assay, Non-Magbeads)
Description: Cytokines play an important role in physiological and pathological processes such as immune regulation, inflammatory response and tumor metastasis. Cytokine detection was wildly used in infectious diseases, autoimmune diseases, tumor auxiliary diagnosis, disease evaluation, medication guidance and prognosis judgment. Multiple cytokines can be detected by the flow cytometry multiplex bead assay at the same time, and multiple results can be obtained in a single sample test at once time, reducing samples and reagents using. The compatible flow cytometers are universal and simple to operate.
Taq Pro Multiplex DNA Polymerase (High sensitivity)
Description: Since December 2019, the emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its associated disease, COVID-19, has caused a devastating pandemic worldwide.As the virus spreads globally, the continuous emergence of new mutant strains escalated the challenge on humans.To facilitate the mutant-related research, drug trials and vaccine development, a multiplex serological test for assessing antibody responses to mutant strains is in urgent need.
VAHTS RNA Multiplex Oligos Set1- Set2 for Illumina
Description: Since December 2019, the emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its associated disease, COVID-19, has caused a devastating pandemic worldwide.As the virus spreads globally, the continuous emergence of new mutant strains escalated the challenge on humans.To facilitate the mutant-related research, drug trials and vaccine development, a multiplex serological test for assessing antibody responses to mutant strains is in urgent need.
Fast Multiplex RT-PCR Kit, 50 reactions/pk, 4 pk/cs
Description: Since December 2019, the emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its associated disease, COVID-19, has caused a devastating pandemic worldwide.As the virus spreads globally, the continuous emergence of new mutant strains escalated the challenge on humans.To facilitate the mutant-related research, drug trials and vaccine development, a high-throughput assay to measure IgG antibodies against the mutants is in urgent need.
Multiplex ELISA Kit For Human Micronutrient Array (7-plex)
Description: Since December 2019, the emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its associated disease, COVID-19, has caused a devastating pandemic worldwide.As the virus spreads globally, the continuous emergence of new mutant strains escalated the challenge on humans.To facilitate the mutant-related research, drug trials and vaccine development, a multiplex serological test for assessing antibody responses to mutant strains is in urgent need.
